RECENT PUBLICATIONS

Conference Proceedings

Conference Report: Challenges
" Outcomes of the Fifth International Conference on Bioscience and Biotechnology, 2024 (ICBB-2024): Planetary Health—A Local Discourse
Suvechhya Bastola, Maria Alba Abad, Anurag Adhikari, Gaurav Adhikari, Aagat Awasthi, Ashim Dhakal, Rijan Maharjan, Rojlina Manandhar, Rukumesh Paudyal, Sunil Pokhrel, Amina Singh, Neha Shrestha, Lekhendra Tripathee, Remco Kort, and Prajwal Rajbhandari

he Fifth International Conference on Bioscience and Biotechnology, 2024 (ICBB-2024), held in Godawari, Nepal, from 21 to 24 April 2024, aimed to address planetary health challenges within the local context of Nepal while fostering global dialogue. Bringing together 240 participants from 10 countries, including 20 international invited speakers, the conference sought to explore the intersections of human health, environmental sustainability, and societal well-being. Jointly organized by the Research Institute for Bioscience and Biotechnology (RIBB), the University of Nepal Development Board (UoN-DB), Vrije Universiteit Amsterdam (VUA), and the Himalayan Environment Research Institute (HERI) and co-organized by Phutung Research Institute (PRI), Kathmandu Research Institute for Biological Sciences (KRIBS), Engage Nepal with Science (ENwS), and Kathmandu Center for Research and Education (KCRE), the conference represented a collaboration of 15 institutions and companies. With attendees from diverse backgrounds—academia, research institutes, private companies, NGOs, and government organizations—the conference featured a robust program of keynotes, workshops, panel discussions, focus group discussions, and oral and poster presentations. Thematic focuses included sessions on Air and Water, Climate Change, Ecology, Evolutionary and Environmental Sciences, and Global Health. A major highlight was the recognition of Nepal’s rich biodiversity and its vulnerability to the impacts of climate change. The event drew inspiration from the European Planetary Health Hub, convening and exploring sustainable, locally relevant solutions to global planetary health issues. Outcomes of the conference included new research collaborations, an enhanced focus on interdisciplinary approaches to biodiversity conservation, and a deeper understanding of how indigenous knowledge can play a crucial role in environmental sustainability. Additionally, ICBB-2024 set a precedent for eco-friendly conferencing practices by emphasizing sustainability throughout the event. In conclusion, ICBB-2024 succeeded in fostering meaningful dialogue and collaboration, inspiring both local and global actions to address pressing planetary health challenges. The event underscored the importance of integrating science, policy, and traditional knowledge in the pursuit of sustainable solutions for planetary health.

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Journal Article

Optica
" When a lensless fluorometer outperforms a lensed system
Asim Maharjan, Prashant Waiba, Shreya Shrestha, Rijan Maharjan, Augusto Martins, Kezheng Li, Emiliano R. Martins, Thomas F. Krauss, and Ashim Dhakal

The excitation and collection of optical signals using lenses form the basis for many applications in imaging, nephelometry, fluorometry, and spectroscopy. While lenses are needed for imaging systems, their use is not so obvious for volume sensing applications. Here, we study the excitation and collection of fluorescence signals to show that lensless systems generally provide a stronger signal compared to lensed systems for the case of extended Lambertian-like sources, such as LEDs. To elucidate this result, we provide a foundational framework to analyze the signal collection efficiency from an arbitrary detection volume with and without lenses when extended sources and detectors are used. A combination of factors, including the limited numerical aperture, the use of extended sources/detectors, and the requirement of a finite imaging distance between the source/detector, lenses, and the sample, limits the performance of the lensed system compared to the lensless system. Our theoretical and experimental results indicate that conventional wisdom based on the assumption of point-like sources and detectors should not always be followed. We provide a systematic approach for analyzing and simplifying the design of low-cost, lensless fluorometers and nephelometers without sacrificing their performance, reporting a sub-ppb level detection limit for measuring tryptophan-like-fluorescence in drinking water.

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Journal Article

Adv. Optical Mater., 2300852. (2023)
" Hybrid Metalens for Miniaturised Ultraviolet Fluorescence Detection
Kezheng Li, Augusto Martins, Sanket Bohora, Ashim Dhakal, Emiliano R. Martins, Thomas Krauss

The advantages of metalenses to enable miniaturized systems have been well established, especially in the visible and infrared wavelength regimes. The ultraviolet (UV) presents a final frontier because feature size scales as the wavelength, so realizing a large size metalens with a high numerical aperture (NA) in the UV is a major challenge. Here, a single-layer, thin-film (450 nm) hybrid metalens with an NA of 0.9 and a diameter of 6.2 mm is presented. By combining a Fresnel lens and optimised binary gratings, the well-known shadowing effect of Fresnel lenses at high NA is avoided while being able to realize a large area lens. It is demonstrated that the combination of high NA and large area affords efficient detection of tryptophan-like fluorescence, which is a well-studied proxy for water contamination with faecal coliforms. The detection of tryptophan at levels better than 1 ppb, which corresponds to the low-risk category for drinking water according to the World Health Organization (WHO), is shown. It is also confirmed that the hybrid metalens fluorescence collection efficiency is 3.5 times higher than a high NA plano-convex lens used in state-of-the-art fluorometers, which demonstrates that the versatile metalens approach opens up new opportunities in the UV.

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